abstract：:The locus responsible for mucoid colony morphology in five independent clinical isolates of Pseudomonas aeruginosa from cystic fibrosis patients have been transferred by means of pM060-mediated conjugation to the genetically characterised strain P. aeruginosa PAO. Genetic mapping has shown that in all five strains the...

journal_title：Journal of medical microbiology

authors： MacGeorge J,Korolik V,Morgan AF,Asche V,Holloway BW

更新日期：1986-06-01 00:00:00

abstract：:The ELISA technique was shown to be group-specific for the detection of IgM antibodies against coxsackie B viruses, and probably against a wider range of enteroviruses. No evidence was obtained that recent coxsackie B-virus infection predisposes to myocardial infarction. ...

journal_title：Journal of medical microbiology

authors： Hannington G,Booth JC,Bowes RJ,Stern H

更新日期：1986-06-01 00:00:00

abstract：:Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

journal_title：Journal of medical microbiology

authors： Tiwari H,Kamat RS

更新日期：1986-05-01 00:00:00

abstract：:The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

journal_title：Journal of medical microbiology

authors： Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

更新日期：1986-05-01 00:00:00

abstract：:The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

journal_title：Journal of medical microbiology

authors： Stevens M,Henrichsen C,Smith M

更新日期：1986-03-01 00:00:00

abstract：:Lincomycin has a differential effect on exoprotein production by Vibrio cholerae. The production of some proteins, such as cholera toxin and deoxyribonuclease, is stimulated by low concentrations of the drug while production of other proteins, such as protease and alkaline phosphatase, is unaffected. Possible mechanis...

journal_title：Journal of medical microbiology

authors： Young DB,Broadbent DA

更新日期：1986-02-01 00:00:00

abstract：:During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

journal_title：Journal of medical microbiology

authors： Lastovica AJ,Le Roux E,Congi RV,Penner JL

更新日期：1986-02-01 00:00:00

abstract：:The encapsulated strain M of Staphylococcus aureus and the encapsulated strain 76 of S. simulans survived incubation with bovine neutrophils in media containing heated bovine serum 0.5% v/v or milk whey 10% v/v. With two unencapsulated strains of S. aureus, M12 and M60, less than 5% of the original inoculum survived. ...

journal_title：Journal of medical microbiology

authors： Anderson JC,Williams MR

更新日期：1985-12-01 00:00:00

abstract：:The effect of pretreatment of denture acrylic with chlorhexidine gluconate on the subsequent adherence of Candida albicans GDH 2346 was measured in vitro. Adherence was significantly reduced by pretreatment with chlorhexidine; maximal inhibition was achieved by incubation at room temperature for 30 min in 2% chlorhexi...

journal_title：Journal of medical microbiology

authors： McCourtie J,MacFarlane TW,Samaranayake LP

更新日期：1985-08-01 00:00:00

abstract：:Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

journal_title：Journal of medical microbiology

authors： Popoff MR,Ravisse P

更新日期：1985-06-01 00:00:00

abstract：:The growth of five species of Bacteroides in four anaerobic culture media was continuously monitored turbidimetrically. Interspecies differences were observed in the growth of Bacteroides spp. in the various media, but growth in Brain Heart Infusion broth supplemented with yeast extract, haemin and menadione, was cons...

journal_title：Journal of medical microbiology

authors： Eley A,Greenwood D,O'Grady F

更新日期：1985-04-01 00:00:00

abstract：:Eleven strains of Mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. Susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. It is concluded that in-vitro inhibition of mycobacte...

journal_title：Journal of medical microbiology

authors： Allen BW

更新日期：1985-04-01 00:00:00

abstract：:The nasal carrier-rate of Staphylococcus aureus in 548 Nigerians aged 9-32 years and attending various educational establishments was 56.4%. This rate decreased with increasing age. A significantly greater proportion of females (65.0%) than males (46.5%) were carriers, but the excess in females was apparent only in su...

journal_title：Journal of medical microbiology

authors： Lamikanra A,Paul BD,Akinwole OB,Paul MO

更新日期：1985-04-01 00:00:00

abstract：:Encapsulation of purified tuberculoproteins in liposomes augmented their ability to elicit delayed hypersensitivity reactions in BCG-immune rats. The effect was most marked with a low-molecular-weight tuberculopeptide that was relatively poor at eliciting reactions when in free form. These findings indicate that, in a...

journal_title：Journal of medical microbiology

authors： Pimm MV,Baldwin RW

更新日期：1984-12-01 00:00:00

abstract：:The faecal flora and mucosa-associated flora (MAF) of rectal biopsy material from 12 patients with active Crohn's disease were studied before and during treatment with a combination of metronidazole and cotrimoxazole given orally for at least 2 weeks. The total faecal flora was greater than the MAF although the propor...

journal_title：Journal of medical microbiology

authors： Hudson MJ,Hill MJ,Elliott PR,Berghouse LM,Burnham WR,Lennard-Jones JE

更新日期：1984-12-01 00:00:00

abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

journal_title：Journal of medical microbiology

authors： Hawkey PM,Bennett PM,Hawkey CA

更新日期：1984-10-01 00:00:00

abstract：:The susceptibility of chicks to enteritis caused by Campylobacter jejuni was studied. Three-day-old chicks did not develop enteritis after oral infection but chicks infected within 12 h of hatching developed gastroenteritis. The incubation period correlated with the inoculum size. Initially, infected chicks developed ...

journal_title：Journal of medical microbiology

authors： Welkos SL

更新日期：1984-10-01 00:00:00

abstract：:The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...

journal_title：Journal of medical microbiology

authors： Sturm AW,Zanen HC

更新日期：1984-10-01 00:00:00

abstract：:The relationship between vaginal carriage and subsequent neonatal acquisition of Clostridium difficile was investigated. Vaginal carriage of C. difficile was detected in 11% of women attending the Department of Genital Medicine Clinic. C. difficile was isolated from the vagina in 18% of 50 mothers before delivery, and...

journal_title：Journal of medical microbiology

authors： Tabaqchali S,O'Farrell S,Nash JQ,Wilks M

更新日期：1984-08-01 00:00:00

abstract：:Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

journal_title：Journal of medical microbiology

authors： Hattikudur S,Kamat RS

更新日期：1984-08-01 00:00:00

abstract：:The determinant responsible for the ability of Bacteroides spp. to inhibit polymorph phagocytic killing of aerobic organisms has not yet been identified. Therefore, the roles of lipopolysaccharide and capsular polysaccharide of B. fragilis were investigated. Serum-resistant and serum-sensitive strains of Proteus mirab...

journal_title：Journal of medical microbiology

authors： Connolly JC,McLean C,Tabaqchali S

更新日期：1984-06-01 00:00:00

abstract：:The effect of various energy sources and metabolic intermediates on the attachment of Treponema pallidum to baby rabbit genital organ (BRGO) cells in culture was examined. Pyruvate and glucose enhanced the motility of T. pallidum in vitro. Pyruvate increased significantly the attachment of treponemes to BRGO cells whe...

journal_title：Journal of medical microbiology

authors： Wong GH,Steiner BM

更新日期：1984-04-01 00:00:00

abstract：:Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...

journal_title：Journal of medical microbiology

authors： Ho-Terry L,Cohen A,Tedder RS

更新日期：1984-02-01 00:00:00

abstract：:In a survey of 3302 people in an area of central Sweden, tinea versicolor was diagnosed in 0.5% of males and 0.3% of females; all those infected were adults. Tinea versicolor is much less common in Sweden than in the tropics. ...

journal_title：Journal of medical microbiology

authors： Hellgren L,Vincent J

更新日期：1983-11-01 00:00:00

abstract：:Urinary isolates of Escherichia coli that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 micrograms/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 micrograms/ml; 73% were multiresistant. Plasmids were detect...

journal_title：Journal of medical microbiology

authors： Kraft CA,Platt DJ,Timbury MC

更新日期：1983-11-01 00:00:00

abstract：:The ability of Proteus mirabilis to produce the bacteriocin, proticine 3, is found almost exclusively in strains that cause severe infections of the upper urinary tract. Proticine 3 was purified from lysates of mitomycin C-induced cultures. Biological activity was associated with structures resembling bacteriophage ta...

journal_title：Journal of medical microbiology

authors： Senior BW

更新日期：1983-08-01 00:00:00

abstract：:One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...

journal_title：Journal of medical microbiology

authors： Copley CG,Egglestone SI

更新日期：1983-08-01 00:00:00

abstract：:Strains of Staphylococcus aureus resistant to gentamicin and methicillin first appeared in Dublin hospitals in 1976, and rapidly became widely disseminated. The number of patients infected or colonised increased throughout the period of study, especially in 1979 and 1980. Most isolates were from burns, surgical wounds...

journal_title：Journal of medical microbiology

authors： Cafferkey MT,Hone R,Falkiner FR,Keane CT,Pomeroy H

更新日期：1983-05-01 00:00:00

abstract：:Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...

journal_title：Journal of medical microbiology

authors： Sprott MS,Ingham HR,Pattman RS,Eisenstadt RL,Short GR,Narang HK,Sisson PR,Selkon JB

更新日期：1983-05-01 00:00:00

abstract：:A collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for Gardnerella vaginalis that could be used in a diagnostic labo...

journal_title：Journal of medical microbiology

authors： Taylor E,Phillips I

更新日期：1983-02-01 00:00:00

abstract：:Lancefield acid extracts of Streptococcus pyogenes, type 22 (T12, M22, OF positive) gave good yields of M protein and little opacity factor (OF), but sodium dodecyl sulphate (SDS) extracts contained high titres of OF (greater than 20000) and little M protein. Acid-extracted OF could be separated from M protein by Seph...

journal_title：Journal of medical microbiology

authors： Hallas G,Widdowson JP

更新日期：1983-02-01 00:00:00

abstract：:Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...

journal_title：Journal of medical microbiology

authors： Dei R,Marmo F,Corte D,Sampietro MG,Franceschini E,Urbano P

更新日期：1982-08-01 00:00:00

abstract：:EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

journal_title：Journal of medical microbiology

authors： Poxton IR,Brown R,Collee JG

更新日期：1982-05-01 00:00:00

abstract：:Transfer of an Hly plasmid determining production of alpha haemolysin to a non-haemolytic strain of Escherichia coli increased the virulence of the strain for mice. Injections of non-toxic amounts of alpha haemolysin, phenylhydrazine, haemoglobin, iron or manganese salts simulated the effect of the Hly plasmid by stim...

journal_title：Journal of medical microbiology

authors： Linggood MA,Ingram PL

更新日期：1982-02-01 00:00:00

abstract：:The identity and motility of enterococci isolated as urinary pathogens were compared with those of strains from other sources. The majority of isolates from all sources proved to be Streptococcus faecalis of S. faecium but eight of 117 urinary strains and seven of 127 non-urinary strains belonged to a group intermedia...

journal_title：Journal of medical microbiology

authors： Bransby RD,Grüneberg RN

更新日期：1982-02-01 00:00:00

abstract：:The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...

journal_title：Journal of medical microbiology

authors： van Dijk WC,Verbrugh HA,van Erne-van der Tol ME,Peters R,Verhoef J

更新日期：1981-11-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...

journal_title：Journal of medical microbiology

authors： Dyson DA,Gilmour NJ,Angus KW

更新日期：1981-02-01 00:00:00

abstract：:A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...

journal_title：Journal of medical microbiology

authors： Kostiala AA

更新日期：1980-05-01 00:00:00

abstract：:A study of 1314 patients with malignancies was made to determine the prevalence of Serratia marcescens in surveillance, diagnostic, and environmental cultures. Sera obtained from a commercial source were used to determine serotypic distributions of the S. marcescens strains isolated during a 51-month period. S. marces...

journal_title：Journal of medical microbiology

authors： Young VM,Moody MR,Morris MJ

更新日期：1980-05-01 00:00:00